Blood tests are the most common diagnostic activity. However, some current blood analysis techniques necessitate large volumes of liquid blood, which can cause fatigue and fainting, and even hospital-acquired anemia in critically ill patients. As liquid, small-volume analysis has not achieved satisfactory accuracy for implementation, solid blood analysis provides a promising alternative method. This study evaluates HemaDrop™, a proprietary analysis technique that solidifies blood drops into thin films on hyper-hydrophilic surfaces. However, concerns inherent in solidifying blood plague the accuracy of traditional solid blood analysis techniques. Specifically, blood can dry non-uniformly, increasing error, as not every spot would have identical composition. Moreover, since blood dries on a substrate, questions about increased drying time and cost. Thus, this research aims to optimize test accuracy, drying time, and cost of samples prepared via HemaDrop™ by manipulating the surface’s hyper-hydrophilicity. This experiment manipulated surface hydrophilicity to find the optimal substrate-coating combination. After two of each combination were prepared, Three-Liquid Contact Angle Analysis characterized one of each pair of samples to quantitatively characterize hydrophilicity. Next, blood was applied to each sample’s counterpart, while qualitative observations coded for markers of disuniformity. Finally, Ion Beam Analysis was performed on two spots of each sample to compare elemental composition and thus measure uniformity. Optimizing HemaDrop™ creates a cost-efficient and accurate method for small-volume blood analysis. By improving the efficiency of blood testing, this research could facilitate a fundamental change to blood analysis and thus could revolutionize patient care.